Insertion junction: CLIP2.050567_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.050567
Chromosome:chromosome 6
Location:8871009
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g311650 IRK1 (1 of 1) PTHR11767//PTHR11767:SF58 - INWARD RECTIFIER POTASSIUM CHANNEL // FI16807P1; Inwardly-rectifying potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAGAGGGACTTGCTGACGCGCTAGAAACGTTTATCGTGCACGCACTA
Internal bar code:AGAAGATCAGTACCCAGGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2065
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGGATAGCGACGAACGAA
Suggested primer 2:CTATGCTACTGGGCTGGAGC