| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050580 |
| Chromosome: | chromosome 9 |
| Location: | 6583349 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416309 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGACCCAGCAGTTCTGGCAGGGGCATACAACACGCTTCGGCGTCAC |
| Internal bar code: | CGTATGGCCTAAGTCTTTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4369 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCCCCACACTTACTCT |
| Suggested primer 2: | TATCCTCAGGTGGAAGGGGG |