Insertion junction: CLIP2.050581_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.050581
Chromosome:plastome
Location:163802
Confidence (%):80
Locus systematic id Locus common name Defline Feature
CreCp.g802324 tic214,orf1995,ycf1,2717035,ChreCp059 Forms with TIC20, TIC56 and TIC100 the 1-MD complex mediating chlorolast protein import CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACCAGATAAACTAATACTTTCATTAACAGCTTTTTGCATTGCTGTTGT
Internal bar code:AGTTGGGGTCTTGCAGTTGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:156
LEAP-Seq percent confirming:25.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCTGCCACATCACAACC
Suggested primer 2:ACTGCACTTGAACCTGTTGGA