Insertion junction: CLIP2.050584_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050584
Chromosome:chromosome 11
Location:2478235
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g095113 DIV24,TFCE1,TFC-E Tubulin-folding cofactor subunit; (1 of 1) IPR000938//IPR029071 - CAP Gly-rich domain // Ubiquitin-related domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCCTACTGCCGACGCGCTCGGCCCTTTCACTGTGCACTGCCCTTCCGA
Internal bar code:GAATATTTTCATGTTGTCAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:679
LEAP-Seq percent confirming:73.3333
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:GACGCTCGGTACAGTGGTAG
Suggested primer 2:CACCATGACCCCACCATGAC