Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.050588 |
Chromosome: | chromosome 2 |
Location: | 6992321 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g389504 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAAGCATGTGCCACACCCTTTGGGCATGCGCGCATGTACAGGCTAAAA |
Internal bar code: | TGTTAAGAACGAATTCATTAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 177 |
LEAP-Seq percent confirming: | 10.5263 |
LEAP-Seq n confirming: | 2 |
LEAP-Seq n nonconfirming: | 17 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGAAGTTGCGCAGAAACACG |
Suggested primer 2: | CAGGGCCCCACTACGTATTC |