| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.050590 |
| Chromosome: | chromosome 12 |
| Location: | 4023424 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516150 | NAT27 | N-acetyltransferase; (1 of 16) 2.3.1.48 - Histone acetyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCATGCAGCTTGGGCCCGGGGCCGGGCAGCAACAGGTCGGCGGG |
| Internal bar code: | TTAGAATGAGCACTTCAATATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3390 |
| LEAP-Seq percent confirming: | 98.6301 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTCCGCACACATTTACA |
| Suggested primer 2: | AGAACATGGGAAGCAGCCTC |