Insertion junction: CLIP2.050594_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050594
Chromosome:chromosome 14
Location:1698726
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g618900 (1 of 2) PF00651//PF07707 - BTB/POZ domain (BTB) // BTB And C-terminal Kelch (BACK) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGAGAAAGTCGAGGAATCCGTTGCCAAACCGACCACCATATCGCTTCG
Internal bar code:CGGGAATTGCTTTTAATGTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGCGCATAAATCCCTGC
Suggested primer 2:CTCCGGACGATACAACTCCG