| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.050604 |
| Chromosome: | chromosome 9 |
| Location: | 4944142 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g403293 | FCL1 | (1 of 1) 6.3.3.2 - 5-formyltetrahydrofolate cyclo-ligase / Methenyl-THF synthetase; 5-Formyltetrahydrofolate cycloligase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTCGTCCTTGCTGCGGGGGATGCGTTTGGGGGCGACGCTGAGCGGG |
| Internal bar code: | GGTAGGTAGGGGTTCCTGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 362 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGGCTCAGGGTATCCAT |
| Suggested primer 2: | CATGCGCTGCAATGAGTTGA |