Insertion junction: CLIP2.050611_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050611
Chromosome:chromosome 6
Location:8395298
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g308000 FAP331 (1 of 3) PTHR32215//PTHR32215:SF0 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 65; Flagellar Associated Protein 331 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTGCCACCCTCCTCCCTACCATCCGAACGCCGTCGTCCTCTCCCATCT
Internal bar code:GTCGTTAGTCCAGTTGCTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:202
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACTCCCCTTCCTCCTGCTC
Suggested primer 2:TTGGAGCAACCTTCCCATCC