Insertion junction: CLIP2.050618_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.050618
Chromosome:chromosome 1
Location:2437813
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g014100 (1 of 1) PTHR14614//PTHR14614:SF29 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // PROTEIN FAM86A intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCAACCTAACCCAGATGATGAGGAGGTGATGGACGCGGCATCAGCCGTC
Internal bar code:GGAGATTCGGCTAGGCATGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:594
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCCATCAAACAACTCCTC
Suggested primer 2:AAACACTTGAAGCGCGCATT