Insertion junction: CLIP2.050641_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.050641
Chromosome:chromosome 10
Location:1593754
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g429050 XPG2 DNA repair 5'-3' exonuclease; (1 of 1) K10846 - DNA excision repair protein ERCC-5 (ERCC5, XPG, RAD2) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCAGATCCCGAGGGACCCGCTCTGAAGCCGGATCCAGACGGACCGGCTCC
Internal bar code:GGAGAAGTTTACTTTCGTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1007
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCCTATACACCCCCAC
Suggested primer 2:CGAGGACGCACACCAAATAC