Insertion junction: CLIP2.050663_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.050663
Chromosome:chromosome 17
Location:2258439
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g713150 FAS8,FLA3 FAS1 domain containing protein;; (1 of 21) PF02469 - Fasciclin domain (Fasciclin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCAGTCCCTCGCCGCCCAGCCCCGAAACCCCGCCGCCCCCTTCGTCG
Internal bar code:TAGTATAGGAAGGAATAGACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:151
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TTCGTCACCCACATGTCCAG
Suggested primer 2:CGCTGTCGTACAAATCGCTG