| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.050667 |
| Chromosome: | chromosome 6 |
| Location: | 5096588 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282651 | TRX16,TRL2 | (1 of 12) 1.6.5.4 - Monodehydroascorbate reductase (NADH); Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCAGCGGTGCAGCGGCGAGCCGTCATCCACCGTCAGGTCCTGCGGCAC |
| Internal bar code: | AGGGCCCGAGGAAAGGAGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 759 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCGCAGATTAGGGGCTG |
| Suggested primer 2: | ATGCTGGTAACACCCCATGG |