| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050672 |
| Chromosome: | chromosome 6 |
| Location: | 8231789 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306550 | (1 of 1) K01166 - ribonuclease T2 [EC:3.1.27.1] (E3.1.27.1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCCTGCAATGCGAAAGCGAGCTCACCCGGCATCCTCCAGCCGAGGGCA |
| Internal bar code: | GGCAGGTGCAAATGCGGCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2594 |
| LEAP-Seq percent confirming: | 96.4286 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGGGCAGACTTTCTTCAC |
| Suggested primer 2: | GGATGGTGAAGTGGAAGGGG |