Insertion junction: CLIP2.050679_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050679
Chromosome:chromosome 16
Location:4920511
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g686750 PTA3 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAGGAGGGGGGAGGGGGGGGGGGGCGCCCAGTGAGGCAGGGAGGCAC
Internal bar code:GTCCTCCAATAGTGGTAATATG

Confirmation - LEAP-Seq

LEAP-Seq distance:908
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCTTTCACACAACCTGC
Suggested primer 2:ACAGGATTGGCAATCAGGGG