Insertion junction: CLIP2.050706_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.050706
Chromosome:chromosome 3
Location:393933
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g144787 TRL12 (1 of 11) 1.8.1.8 - Protein-disulfide reductase / Protein disulfide reductase; Thioredoxin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGGCGCTGGCTGAGGACGTGCGAAAGCTCAAGTCCACATTCAGGGAGG
Internal bar code:GGTAGACGTGACAATAGCCAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1847
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:ACGACATCAACGCTGTGCTA
Suggested primer 2:GTGTACACGATGAATGCCGC