| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050738 |
| Chromosome: | chromosome 5 |
| Location: | 3451389 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233303 | (1 of 1) K11718 - UDP-glucose:glycoprotein glucosyltransferase (HUGT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAACACCCCTGGCCCATCCCGAGCCTGCTGTACCACTGCGCCTGTCC |
| Internal bar code: | GCCTACGCTTGGGGACCCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTCGTTTTCGTTGGTTT |
| Suggested primer 2: | ACCGCTTTTCCAACCATCCT |