| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050766 |
| Chromosome: | chromosome 10 |
| Location: | 6241159 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463250 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGAACTACATGACGCCCACCAGAATGCCGTTTGCCCCCACACCCCAC |
| Internal bar code: | GAATGAGGTAACAAGGATAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2075 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGACGAGGTCAGCAGAT |
| Suggested primer 2: | CATCCCCCAATTCCCTGTCC |