| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.050775 |
| Chromosome: | chromosome 17 |
| Location: | 2314175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713600 | SUB13 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGTCGCCTAGGTCGTGGTGCTCAGCCACCGCCTGCTAAGGCGCTGAT |
| Internal bar code: | CGGTTTATTCTTAACGCACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1535 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCGTGTGGATGGCATG |
| Suggested primer 2: | CCCCTATCCCAAACCCAACC |