| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050789 |
| Chromosome: | chromosome 16 |
| Location: | 792714 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690700 | (1 of 13) IPR000086//IPR015797 - NUDIX hydrolase domain // NUDIX hydrolase domain-like | intron | |
| Cre16.g690750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCTGCAAGCTATAGGATGCACGTCAAGGCGGCTCGCCAAGCCACCAT |
| Internal bar code: | TTAGAAAGGTCAAACAGGCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3546 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 85 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGCCGCAACAAGTTCAAG |
| Suggested primer 2: | GTGGTGTAGTGTGGATGGGG |