| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050795 |
| Chromosome: | chromosome 3 |
| Location: | 559231 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145267 | (1 of 1) 3.5.4.3 - Guanine deaminase / Guanine aminase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCCCCTACAAATGTACGACAGCCTCGGGCCTAGGTCTCATCACACG |
| Internal bar code: | GCCGGTCTTTAAATCCATGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1393 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAAGACGAGTGTGCGTGT |
| Suggested primer 2: | TCAAGTTCAAAGCGCGGTTG |