Insertion junction: CLIP2.050809_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050809
Chromosome:chromosome 6
Location:3628037
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278138 OPR119 OctotricoPeptide Repeat protein 119, pseudogene; (1 of 781) IPR000104 - Antifreeze protein, type I intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGTAGGGGGGTCGTAGATACCAGCCCAAACTATTCCGAACCCAATGG
Internal bar code:TATCACCTACTAAGCTAAAGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1620
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCTGGATCCAAGAAGCA
Suggested primer 2:GGGAATGGGGAACTTGAGGG