| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.050812 |
| Chromosome: | chromosome 1 |
| Location: | 2670848 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016100 | (1 of 7) IPR011048 - Cytochrome cd1-nitrite reductase-like, haem d1 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCAGGTGCGACGACGACTACGTGTGTGCGTGTGCAGTGCTAGTGTG |
| Internal bar code: | GAGAGAAGGCACCTGTCGTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2886 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGGGCAATCAAGGGGAG |
| Suggested primer 2: | GTACCACGCACGGTATCGAT |