Insertion junction: CLIP2.050815_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.050815
Chromosome:chromosome 16
Location:1983220
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g656500 ALA3 (1 of 1) K01530 - phospholipid-translocating ATPase (E3.6.3.1); ATPase, phospholipid transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCTCCGCCGCCGACCACGTCTACGGCAAGAAGACCTGCGAGTGAGTATT
Internal bar code:CAAGTAGCTCGTCATGCTTTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:327
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACGACCCACACAGTCATCA
Suggested primer 2:AGTACCCGGACTTCTACCCC