Insertion junction: CLIP2.050830_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.050830
Chromosome:chromosome 3
Location:7713596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g203650 (1 of 1) IPR009072//IPR013785 - Histone-fold // Aldolase-type TIM barrel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCACCACCACCAGCCCCCACTGGCGGAGCAACTGCGTGAACTGCTGCAC
Internal bar code:CGACCACAACATCACGACAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:4224
LEAP-Seq percent confirming:90.1639
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTCTGATCCCCATCGACG
Suggested primer 2:GACGACCGACCCTGAACTTT