Insertion junction: CLIP2.050830_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.050830
Chromosome:chromosome 3
Location:7713596
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g203650 (1 of 1) IPR009072//IPR013785 - Histone-fold // Aldolase-type TIM barrel CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAGAGATGAGAAGAGATAGGCTGAGAGGTTGCGGACAGTTGTGCGGAG
Internal bar code:GGGGGCGCTAGGGTCAATTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2538
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GACGACCGACCCTGAACTTT
Suggested primer 2:CAGTCTGATCCCCATCGACG