| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.050832 |
| Chromosome: | chromosome 9 |
| Location: | 2785183 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391150 | DIV153 | (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGAGGGCTCCCCTCCTCCTCGCAGTAAGTAGCTACATGCGCCGTCAA |
| Internal bar code: | TTATATGTTCTCCTTATCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1713 |
| LEAP-Seq percent confirming: | 96.9697 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCTTCGAGACCAAGGACC |
| Suggested primer 2: | ATGGACAACGACATCCCCAC |