| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050837 |
| Chromosome: | chromosome 8 |
| Location: | 300350 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358569 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCTCATCCCCAGTACCGGTAGCCTCCCTCCCATGCATTACGCCCAGAC |
| Internal bar code: | CTACGCCAATACCAGTGTGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 487 |
| LEAP-Seq percent confirming: | 23.7288 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACACAGCACGGTAACAG |
| Suggested primer 2: | CAAACCCACTGTTGACGCAG |