| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.050848 |
| Chromosome: | chromosome 10 |
| Location: | 4447856 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g450650 | (1 of 34) PF00168 - C2 domain (C2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGTGCATGCGTGTGTGTGTGGGCGTGTGTGTGGGGGGGGGCGTCCTC |
| Internal bar code: | TATCGTGAAGATACCTCATCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1845 |
| LEAP-Seq percent confirming: | 54.8387 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACCCTGCCAAGATGCGTT |
| Suggested primer 2: | ATGCGTGTGTGTGGGTATGT |