| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.050849 |
| Chromosome: | chromosome 12 |
| Location: | 6533354 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538000 | EZY5 | Early zygote expressed protein; (1 of 26) IPR001202 - WW domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGCCTTCAGGCTGGGAGGCTCCAGGGGAATCGTGATGTCAACGTTGT |
| Internal bar code: | GTATGCTATGAGCGCGTAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2937 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGAGTCATATCAGCAGC |
| Suggested primer 2: | CCCATTGCCCAGAATCCACT |