Insertion junction: CLIP2.050864_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.050864
Chromosome:chromosome 15
Location:1456961
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g641800 RABE1,RAB8 RAB-Like GTP binding Protein; (1 of 1) K07901 - Ras-related protein Rab-8A (RAB8A, MEL) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCCGCTCAACAGATCCTGGTGGGCAACAAGCTGGACCTGTCCGAAAAG
Internal bar code:GGGTGGCTTTTGACTATACACA

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATACGCACGCACACTCATG
Suggested primer 2:GTGGACGTCGAGGGAAAACT