Insertion junction: CLIP2.050879_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.050879
Chromosome:chromosome 10
Location:4646744
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g451752 PHC25 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGGTAAGATCCCGTTGTGTCCTGTGACCTCTAATCCAACAGCCCTCCC
Internal bar code:ATACGATCTCGTTCTTGCTCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1188
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAGCGCGGTCAGTTACA
Suggested primer 2:CTAACCCTCCCCCTCCTCC