| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.050886 |
| Chromosome: | chromosome 16 |
| Location: | 3053375 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664250 | OASTL2,ASL2 | O-acetylserine (Thiol)-lyase/cysteine synthase 2; (1 of 1) 4.2.1.22 - Cystathionine beta-synthase / Serine sulfhydrase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTCCCTACCCACCTACGCACACGTACCCGTCTTATTTGCCGTCTGC |
| Internal bar code: | GTCCGAGTCTCGGCTAGCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4241 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGTTTGGGCTACCGTG |
| Suggested primer 2: | TTCTTCTTGAGGCGCCTCTG |