Insertion junction: CLIP2.050933_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.050933
Chromosome:chromosome 9
Location:3120242
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394251 PF16 Central pair associated protein; (1 of 1) PF00514//PF13513 - Armadillo/beta-catenin-like repeat (Arm) // HEAT-like repeat (HEAT_EZ) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCATGTCGACGTGTCCAACAGTTGGGCACCCAGTCTTTCGTCGCTGA
Internal bar code:CCTGGTGAGATAAATACGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1869
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTTGGAGATGTCGGACA
Suggested primer 2:AGGTTTTCCCGCTCTAACCG