Insertion junction: CLIP2.050940_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.050940
Chromosome:chromosome 7
Location:5979668
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g354551 FAP65 (1 of 2) PTHR10270:SF161 - NUCLEAR CONTROL OF ATPASE PROTEIN 2; Flagellar Associated Protein 65 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTCCGTGCTTAAGATTCGCTTCAAGCAGCCCACCAGCAAAGCCTTCG
Internal bar code:CTTCTAAGGACATGGGCACCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:5544
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGGACGTCGCATTTCATG
Suggested primer 2:AAACGCTTTCGCTGGTGTTC