| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.050945 |
| Chromosome: | chromosome 2 |
| Location: | 5831577 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g117200 | Putative beta-galactosidase; (1 of 5) 3.2.1.23 - Beta-galactosidase / Lactase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGGGCCGTTGCCTGCTGCCGAGTATGTGCTGCGTGTGATAAGACCTT |
| Internal bar code: | GCTAGTGATTCAAACTCCCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1792 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCGTATTTTGGGAAGCAT |
| Suggested primer 2: | TCTAACAAAATCGCGGCCCT |