Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.050954 |
Chromosome: | chromosome 17 |
Location: | 649924 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre17.g700200 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACGCCTGGCGCTGAGCTCCTACTGCCATCCTCGTGTGTGTCGTGCTAC |
Internal bar code: | CCCCAATAATAGTGCACTAGCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1176 |
LEAP-Seq percent confirming: | 69.697 |
LEAP-Seq n confirming: | 23 |
LEAP-Seq n nonconfirming: | 10 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGTAGCCTTTGACCACCAC |
Suggested primer 2: | GAAGGCGCCCATGTCAATTC |