Insertion junction: CLIP2.050965_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.050965
Chromosome:chromosome 14
Location:2436287
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g623650 ADH7 Alcohol dehydrogenase; (1 of 3) 1.1.1.2 - Alcohol dehydrogenase (NADP(+)) / Aldehyde reductase (NADPH) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAGCAGGCGACCAGCCACCAGGTGCGACTGTCCCCTGTGACGACATGGC
Internal bar code:CTTTTACCGGTCGAATATTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:730
LEAP-Seq percent confirming:26.6667
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGATCTCACTGGGTCCCA
Suggested primer 2:TCGGCTAACGCTTTCCCATT