Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | + |
Strain: | CLIP2.050969 |
Chromosome: | chromosome 2 |
Location: | 7387217 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre09.g391986 | (1 of 1) 3.1.1.32 - Phospholipase A(1) / Phospholipase A1 | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGACAGCCTGATACCGGGCTCGCTGGCCTCGCAGCTCCTGGCAGGTA |
Internal bar code: | CTAGCATAGCAAATTCTCAATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3583 |
LEAP-Seq percent confirming: | 95.4545 |
LEAP-Seq n confirming: | 63 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCTCCACGATCCAGTTGTG |
Suggested primer 2: | ACCAGCCTCATCGACTACCT |