Insertion junction: CLIP2.050979_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.050979
Chromosome:chromosome 1
Location:1467385
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g007811 (1 of 1) PF15383 - Transmembrane protein 237 (TMEM237) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAAAGGAGGCGCGAATGCAGTCCAGCCCCACCCCTCCCACCCCACAGC
Internal bar code:TTTGGTGTGTAGAATTTCTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:18.1818
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACCTGTCTCTCCC
Suggested primer 2:CTCATGGCATTGTGACCCCT