| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.050991 |
| Chromosome: | chromosome 6 |
| Location: | 6179915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g291950 | MRS4,MRS3 | Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGTGGGTGCTGCTAGGACCACCGGGCCCCGGTCAACACAATGTTATT |
| Internal bar code: | CATAGTACCATAGGGGTCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1239 |
| LEAP-Seq percent confirming: | 23.1343 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTGGACATACAGGTGT |
| Suggested primer 2: | GCCCCTCATACACACACACA |