Insertion junction: CLIP2.051006_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.051006
Chromosome:chromosome 11
Location:849359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467645 (1 of 2) PTHR10540:SF8 - COP9 SIGNALOSOME COMPLEX SUBUNIT 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCAACCACCCACCGAACAGGAGTACAACGACACGCTGCTCACCCTCTA
Internal bar code:TTTTGTGTATCATCCGACAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2939
LEAP-Seq percent confirming:91.2281
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTCCTTTGGGAGGCGATG
Suggested primer 2:GTATGGAAACCGGTGGACGT