| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051010 |
| Chromosome: | chromosome 6 |
| Location: | 3215948 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276600 | HTR2 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCGTGACACGCTTGGCGTGGATGGCGCACAGGTTGGTGTCCTCGAACA |
| Internal bar code: | GTCTTATGAGGTACTTAATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2512 |
| LEAP-Seq percent confirming: | 90.8397 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGCCCTTTTATTGGGGT |
| Suggested primer 2: | TCTGGCTGTGATTGAGCAGG |