Insertion junction: CLIP2.051016_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.051016
Chromosome:chromosome 11
Location:3340079
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g476700 MPA9 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGTGCGACACGATACGCCCAAACAAAACAACAAACAAAACAGAACAGAG
Internal bar code:CTTAGTTTCCCCATTATTAGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:290
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGTCAACTCCAGCGACA
Suggested primer 2:CACACACAACAGCACAGCTC