Insertion junction: CLIP2.051019_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.051019
Chromosome:chromosome 1
Location:4025514
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g026500 ASP3 (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCCGTACGCCGTCAGGAAGCTTGTGATGCCTGACGGGCAGAGCAAAC
Internal bar code:GATGTGTTCACTGAGTCTCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:892
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGACTTGTGGGCCTTTGC
Suggested primer 2:CTTCCCTCCACTGCGGAAAT