Insertion junction: CLIP2.051030_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.051030
Chromosome:chromosome 2
Location:6992950
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389504 (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) CDS
Cre09.g389541 OPR9 OctotricoPeptide Repeat protein 9; (1 of 1) IPR000104//IPR011237//IPR011335//IPR013584 - Antifreeze protein, type I // Peptidase M16 domain // Restriction endonuclease type II-like // RAP domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGCGCGATGTCCTCAACCAGAAAGCAACAAGCGGGCTCGCATGCGCA
Internal bar code:ATAGCGTCATCTAGGTTGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:820
LEAP-Seq percent confirming:81.0811
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGTAAACCAAGAGGAGGC
Suggested primer 2:AAATTGCGCAACCAGCCAAT