| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051035 |
| Chromosome: | chromosome 9 |
| Location: | 2999684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393506 | HCP3 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGTGATTCGCTCAATTCCTGTAAATCTTGCCTACCGTGACTCCTGT |
| Internal bar code: | GTAAGCGGCAGCTCCGACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3145 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAGGCCCCTCCTAATT |
| Suggested primer 2: | TTTGTTGCATTCGACGGCAG |