| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.051057 |
| Chromosome: | chromosome 10 |
| Location: | 751151 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422700 | SMM35 | (1 of 25) IPR013216//IPR029063 - Methyltransferase type 11 // S-adenosyl-L-methionine-dependent methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCTTAGGGCGCAGCTAATTCCCATACCCAACAAATGGCAAACAATTG |
| Internal bar code: | ACATTCCGTGTTGTCCATAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1005 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCAAATGAGCGAGG |
| Suggested primer 2: | CTGGCTACTGCTGGCTACTG |