Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.051082 |
Chromosome: | chromosome 3 |
Location: | 4330591 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g174500 | HDZ1 | (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCGGACAGCGGCCCCGTGTCGGCGCTGAAGCGCGACCTGCTGCGCC |
Internal bar code: | CGGGTGATACGGGCTCACGGTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 320 |
LEAP-Seq percent confirming: | 76.9231 |
LEAP-Seq n confirming: | 10 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCAGCCAGTTATCTCACGCA |
Suggested primer 2: | AACCGCTACTGGCTCTTCAC |