Insertion junction: CLIP2.051082_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.051082
Chromosome:chromosome 3
Location:4330591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g174500 HDZ1 (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGGCGGACAGCGGCCCCGTGTCGGCGCTGAAGCGCGACCTGCTGCGCC
Internal bar code:CGGGTGATACGGGCTCACGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGCCAGTTATCTCACGCA
Suggested primer 2:AACCGCTACTGGCTCTTCAC