| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.051082 |
| Chromosome: | chromosome 3 |
| Location: | 4330593 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g174500 | HDZ1 | (1 of 1) PF00046 - Homeobox domain (Homeobox); Zygote-specific homeobox protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATCCAGCCCCTCCGTGTAGTCGTCGTCATCGGCTGCCAGCACCGCAG |
| Internal bar code: | ATTGGTTTGGGTTATTAGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1334 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGCTACTGGCTCTTCAC |
| Suggested primer 2: | CCAGCCAGTTATCTCACGCA |